Por favor, use este identificador para citar o enlazar este ítem:
https://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/12316Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.contributor.author | Gutiérrez Bermón, Karen Juliana. | - |
| dc.date.accessioned | 2026-11-09T16:55:54Z | - |
| dc.date.available | 2025 | - |
| dc.date.available | 2026-11-09T16:55:54Z | - |
| dc.date.issued | 2025 | - |
| dc.identifier.citation | Gutiérrez Bermón, K. J. (2025). Descripción de lesiones y estandarización de una técnica molecular para el diagnóstico de Flavobacterium psychrophilum en trucha arcoíris (Oncorhynchus mykiss) [Trabajo de Grado Pregrado, Universidad de Pamplona]. Repositorio Hulago Universidad de Pamplona. https://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/12316 | es_CO |
| dc.identifier.uri | https://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/12316 | - |
| dc.description | Flavobacterium psychrophilum es una bacteria que afecta salmónidos y otras especies piscícolas incluyendo la trucha arcoíris (Oncorhynchus mykiss), causando la enfermedad bacteriana del agua fría (BCWD), caracterizada por lesiones dérmicas, exoftalmia, palidez branquial e incluso deformidades a nivel esquelético como escoliosis. En este estudio, se describen las lesiones compatibles con Flavobacterium spp y las pruebas iniciales para la estandarización de una prueba de PCR para la detección molecular de F. psychrophilum a partir de 33 muestras de tejido provenientes de ejemplares de truchícolas de Mutiscua y Pamplona. Inicialmente se evaluaron dos componentes diferentes para los tampones de lisis (urea y SDS) utilizados en la extracción de ADN, observando que el buffer con urea obtuvo mayores concentraciones de ADN, aunque presentó limitaciones durante la extracción con fenol-cloroformo debido a la inversión de fases causada por su elevada densidad. Se realizó una PCR anidada usando como cebadores externos 20F/1500R que amplifican una secuencia ribosomal 16S y como cebadores internos PSY1/PSY2, específicos para determinar F. psycrophilum optimizando la temperatura de anillamiento (Tm) a 55 °C, con la cual se obtuvo mejores resultados en cuanto a especificidad de amplificación. Adicionalmente, se desarrolló una PCR sobre muestras de bacterias aisladas en campo, encontrado solo amplificaciones de los genes ribosomales. Este trabajo representa un primer acercamiento y un importante aporte a la obtención de un método molecular para el diagnóstico de la enfermedad del agua fría que es un problema importante en las explotaciones truchícolas de la región. | es_CO |
| dc.description.abstract | Flavobacterium psychrophilum is a bacterium that affects salmonids and other fish species, including rainbow trout (Oncorhynchus mykiss), causing bacterial cold water disease (BCWD), characterized by skin lesions, exophthalmos, gill paleness, and even skeletal deformities such as scoliosis. This study describes lesions consistent with Flavobacterium spp. and the initial tests for standardizing a PCR assay for the molecular detection of F. psychrophilum from 33 tissue samples obtained from rainbow trout in Mutiscua and Pamplona. Two different components were initially evaluated for the lysis buffers (urea and SDS) used in DNA extraction. The urea buffer yielded higher DNA concentrations, although it presented limitations during phenol-chloroform extraction due to phase inversion caused by its high density. A nested PCR was performed using as external primers the 20F/1500R primers, which amplify a 16S ribosomal sequence, and the PSY1/PSY2 as internal primers, specific for detecting F. psychrophilum. The annealing temperature (Tm) was optimized at 55°C, which yielded the best results in terms of amplification specificity. Additionally, a PCR was performed on samples of bacteria isolated in the field, revealing amplifications only of ribosomal genes. This work represents a first step and an important contribution to obtaining a molecular method for diagnosing cold-water disease, a significant problem in trout farms in the region. | es_CO |
| dc.format.extent | 61 | es_CO |
| dc.format.mimetype | application/pdf | es_CO |
| dc.language.iso | es | es_CO |
| dc.publisher | Universidad de Pamplona - Facultad de Ciencias Agrarias. | es_CO |
| dc.subject | Estandarización. | es_CO |
| dc.subject | Fenol-cloroformo. | es_CO |
| dc.subject | Ulceras. | es_CO |
| dc.subject | Cebadores. | es_CO |
| dc.subject | PCR anidada. | es_CO |
| dc.title | Descripción de lesiones y estandarización de una técnica molecular para el diagnóstico de Flavobacterium psychrophilum en trucha arcoíris (Oncorhynchus mykiss). | es_CO |
| dc.type | http://purl.org/coar/resource_type/c_7a1f | es_CO |
| dc.date.accepted | 2025 | - |
| dc.relation.references | Abm. (s. f.). Polymerase Chain Reaction (PCR) - Variations to the System. Tomado de https://info.abmgood.com/polymerase-chain-reaction-pcr-variations. | es_CO |
| dc.relation.references | Alcántara-Jauregui, F. M., Martínez-Salazar, E. A., & Contreras-Rendón, S. (2022). Enfermedades bacterianas y sus agentes etiológicos en acuicultura de agua dulce en Colombia. Revista MVZ Córdoba, 27(3), e2387. https://revistamvz.unicordoba.edu.co/article/view/2387/4753. | es_CO |
| dc.relation.references | Amillano-Cisneros, J. M., Fuentes-Valencia, M. A., Leyva-Morales, J. B., Savín-Amador, M., Márquez-Pacheco, H., Bastidas-Bastidas, P. d. J., Leyva-Camacho, L., De la Torre-Espinosa, Z. Y., & Badilla-Medina, C. N. (2025). Effects of Microorganisms in Fish Aquaculture from a Sustainable Approach: A Review. Microorganisms, 13(3), 485. https://doi.org/10.3390/microorganisms13030485. | es_CO |
| dc.relation.references | Baliarda, A., Faure, D., & Urdaci, M. C. (2002). Development and application of a nested PCR to monitor brood stock salmonid ovarian fluid and spleen for detection of the fish pathogen Flavobacterium psychrophilum. Journal of applied microbiology, 92(3), 510–516. https://doi.org/10.1046/j.1365-2672.2002.01554.x. | es_CO |
| dc.relation.references | Barnes, M. E. & Brown, M. L. (2011). A review of Flavobacterium psychrophilum biology, clinical signs, and bacterial cold water disease prevention and treatment. The Open Fish Science Journal, 4: 40-48. http://dx.doi.org/10.2174/1874401X01104010040. | es_CO |
| dc.relation.references | Bernardet, J. F., Nakagawa, Y., Holmes, B., & Subcommittee On The Taxonomy Of Flavobacterium And Cytophaga-Like Bacteria Of The International Committee On Systematics Of Prokaryotes (2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. International journal of systematic and evolutionary microbiology, 52(Pt 3), 1049–1070. https://doi.org/10.1099/00207713-52-3-1049. | es_CO |
| dc.relation.references | Bernardet, J.F. & Grimont, P.A. (1989). Deoxyribonucleic Acid Relatedness and Phenotypic Characterization of Flexibacter columnaris sp. nov., nom. rev., Flexibacter psychrophilus sp. nov., nom. rev., and Flexibacter maritimus Wakabayashi, Hikida, and Masumura. Microbiology Society. Vol 39. https://doi.org/10.1099/00207713-39-3-346. | es_CO |
| dc.relation.references | Bernardet, J.F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K., Vandamme, P., (1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). International Journal of Systematic and Evolutionary Microbiology. 46, 128–148. https://doi.org/10.1099/00207713-46-1-128. | es_CO |
| dc.relation.references | Burgos, A. (2006). Detección molecular de flavobacterium psychrophilumen trucha arco iris (Oncorhynchus mykiss) cultivada en el lago Guamuéz municipio de Pasto República de Colombia. Revista electrónica de ingeniería en producción acuícola Vol 2. Recuperado de https://revistas.udenar.edu.co/index.php/reipa/article/view/1617/1975. | es_CO |
| dc.relation.references | Castro-Rojas, C., Tere-Peña, C., Arias-Cortés, M. (2023). Guide on Polymerase Chain Reaction (PCR) for assuring validity of its results. Instituto Nacional de Metrología. 132 pp. https://doi.org/10.13140/RG.2.2.14178.24009. | es_CO |
| dc.relation.references | Cipriano, R. C. & Holt, R. A. (2005). Flavobacterium psychrophilum, cause of Bacterial Cold-Water Disease and Rainbow Trout Fry Syndrome. Fish Disease Leaflet. No. 86. Recuperado de https://freshwater-aquaculture.extension.org/wp-content/uploads/2019/08/USFWS_Coldwater_disease.pdf. | es_CO |
| dc.relation.references | Crumlish, M., Diab, A. M., George, S., & Ferguson, H. W. (2007). Detection of the bacterium Flavobacterium psychrophilum from a natural infection in rainbow trout, Oncorhynchus mykiss (Walbaum), using formalin-fixed, wax-embedded fish tissues. Journal of fish diseases, 30(1), 37–41. https://doi.org/10.1111/j.1365-2761.2007.00779.x. | es_CO |
| dc.relation.references | Crump, E. M., Burian, J., Allen, P. D., & Kay, W. W. (2005). Identification and expression of a host-recognized antigen, FspA, from Flavobacterium psychrophilum. Microbiology (Reading, England), 151(Pt 9), 3127–3135. https://doi.org/10.1099/mic.0.28140-0. | es_CO |
| dc.relation.references | Dalsgaard, I. & Madsen, L. (2000). Bacterial pathogens in rainbow trout, Oncorhynchus mykiss (Walbaum), reared at Danish freshwater farms. Journal of Fish Diseases, 23: 199-209. https://doi.org/10.1046/j.1365-2761.2000.00242.x. | es_CO |
| dc.relation.references | Dalsgaard, I. 1993. Virulence mechanisms in Cytophaga psychrophila and other Cytophaga-like bacteria pathogenic for fish. Annual Review of Fish Diseases. 3: 127-144. https://doi.org/10.1016/0959-8030(93)90032-7. | es_CO |
| dc.relation.references | Duman, M., Altun, S., Saticioglu, I. B., & Romalde, J. L. (2025). A review of bacterial disease outbreaks in rainbow trout (Oncorhynchus mykiss) reported from 2010 to 2022. Journal of fish diseases, 48(9), e13886. https://doi.org/10.1111/jfd.13886. | es_CO |
| dc.relation.references | Easy, R. H., & Ross, N. W. (2010). Changes in Atlantic salmon Salmo salar mucus components following short- and long-term handling stress. Journal of fish biology, 77(7), 1616–1631. https://doi.org/10.1111/j.1095-8649.2010.02796.x. | es_CO |
| dc.relation.references | European Market Observatory for Fisheries and Aquacukture Products (EMOFA). (2023). Large Trout In The EU. Tomado de https://eumofa.eu/documents/20124/35623/PTAT_Large%2Btrout.pdf/4923045b-73a7-e7ce-16ec-bd33301286a8?t=1686569699668. | es_CO |
| dc.relation.references | Foster, B. (2024). A Guide to DNA and RNA Quantification and Quality. Technology Networks. Tomado de https://www.technologynetworks.com/genomics/articles/a-guide-to-dna-and-rna-quantification-and-quality-392900. | es_CO |
| dc.relation.references | Grasteau, A., Guiraud, T., Calvez, S., Chesneau, V., Le Hénaff, M., et al. (2015). Evaluation of Glutaraldehyde, Chloramine-T, Bronopol, Incimaxx Aquatic and Hydrogen Peroxide as Biocides against Flavobacterium psychrophilum for Sanitization of Rainbow Trout Eyed Eggs. Journal of Aquaculture Research and Development. 6. 6-13. http://dx.doi.org/10.4172/2155-9546.1000382. | es_CO |
| dc.relation.references | Herzer, S. (2001). DNA purification. En Gerstein, A. S. (Ed.), Molecular biology problem solver: A laboratory guide. Wiley-Liss pp. 167-195. https://doi.org/10.1002/0471223905.ch7. | es_CO |
| dc.relation.references | Instituto Colombiano Agropecuario (ICA). (2018). El ICA atendió la notificación de mortalidad de trucha arcoíris en Nariño. Recuperado de https://www.ica.gov.co/noticias/ica-notificacion-mortalidad-trucha-arcoiris. | es_CO |
| dc.relation.references | Izumi, S., & Wakabayashi, H. (1997). Use of PCR to detect Cytophaga psychrophila from apparently healthy Juvenile ayu and coho salmon eggs. Fish Pathology, 32(3), 169-173. https://doi.org/10.3147/jsfp.32.169. | es_CO |
| dc.relation.references | Izumi, S., Fujii, H., & Aranishi, F. (2005). Detection and identification of Flavobacterium psychrophilum from gill washings and benthic diatoms by PCR-based sequencing analysis. Journal of fish diseases, 28(9), 559–564. https://doi.org/10.1111/j.1365-2761.2005.00663.x. | es_CO |
| dc.relation.references | Johansen, L., Jensen, I., Mikkelsen, H., Bjørn, P., Jansen, P., & Bergh, O. (2011). Disease interaction and pathogens exchange between wild and farmed fish populations with special reference to Norway. Aquaculture, 315(3–4), 167–186. https://doi.org/10.1016/j.aquaculture.2011.02.014. | es_CO |
| dc.relation.references | Knupp, C., & Loch, T. P. (2025). Immersion challenge of three salmonid species (family Salmonidae) with three multilocus sequence typing variants of Flavobacterium psychrophilum provides evidence of differential host specificity. Journal of fish diseases, 48(8), e13889. https://doi.org/10.1111/jfd.13889. | es_CO |
| dc.relation.references | Köchl, S., Niederstätter, H., Parson, W. (2005). DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR. In: Carracedo, A. (eds) Forensic DNA Typing Protocols. Methods in Molecular Biology, vol 297. Humana Press. https://doi.org/10.1385/1-59259-867-6:013. | es_CO |
| dc.relation.references | León, J., Ávalos, R., & Ponce, M. (2008). Flavobacterium psychrophilum y su patología en alevines de Onchorhynchus mykiss del centro piscícola El Ingenio, Huancayo. Revista Peruana de Biología, 15(2), 117-124. Recuperado de http://www.scielo.org.pe/scielo.php?script=sci_arttext&pid=S1727-99332008000200019&lng=es&tlng=es. | es_CO |
| dc.relation.references | Lorenzen, E., Dalsgaard, I., Bernardet, J. (1997). Characterization of isolates of Flavobacterium psychrophilum associated with coldwater disease or rainbow trout fry syndrome I: phenotypic and genomic studies. Dis Aquat Org. 31:197- 208. https://doi.org/10.3354/dao031197. | es_CO |
| dc.relation.references | Lumsden, J. S., Ostland, V. E. & Ferguson, H. W. (1996). Necrotic myositis in cage cultured rainbow trout, Oncorhynchus mykiss (Walbaum), caused by Flexibacter psychrophilus. Journal of Fish Diseases 19: 113–119. https://doi.org/10.1111/j.1365-2761.1996.tb00689.x. | es_CO |
| dc.relation.references | Madetoja, J., Dalsgaard, I., & Wiklund, T. (2002). Occurrence of Flavobacterium psychrophilum in fish-farming environments. Diseases of aquatic organisms, 52(2), 109–118. https://doi.org/10.3354/dao052109. | es_CO |
| dc.relation.references | Madsen, L. & Dalsgaard, I. (1999). Vertebral column deformities in farmed rainbow trout (Oncorhynchus mykiss). Aquaculture. 171: 41–48. https://doi.org/10.1016/S0044-8486(98)00427-X. | es_CO |
| dc.relation.references | Madsen, L. J. Arnbjerg, and I. Dalsgaard. (2001). Radiological examination of the spinal column in farmed rainbow trout Oncorhynchus mykiss (Walbaum): experiments with Flavobacterium psychrophilum and oxytetracycline. Aquacult. Res. 32: 235–241. https://doi.org/10.1046/j.1365-2109.2001.00552.x. | es_CO |
| dc.relation.references | Madsen, L. J. Arnbjerg, and I. Dalsgaard. (2001). Radiological examination of the spinal column in farmed rainbow trout Oncorhynchus mykiss (Walbaum): experiments with Flavobacterium psychrophilum and oxytetracycline. Aquacult. Res. 32: 235–241. https://doi.org/10.1046/j.1365-2109.2001.00552.x. | es_CO |
| dc.relation.references | Mancera, L. P., Gordillo, R. F., Albarracín, N., y Piza, A. C. (2025). Certificación kosher en la industria acuícola colombiana: Caso de implementación en la trucha arcoíris (Onchorhynchus mykiss). Región Científica, 4(2), 2025505. https://doi.org/10.58763/rc2025505. | es_CO |
| dc.relation.references | Matlock, B. (2015). Assessment of Nucleic Acid Purity. Thermo Fisher Scientific. Recuperado de https://documents.thermofisher.com/TFS-Assets/CAD/Product-Bulletins/TN52646-E-0215M-NucleicAcid.pdf#:~:text=A%20low%20A260%2FA230%20ratio%20may,the%20same%20pH%20and%20of. | es_CO |
| dc.relation.references | Matlock, B. (2015). Assessment of Nucleic Acid Purity. Thermo Fisher Scientific. Recuperado de https://documents.thermofisher.com/TFS-Assets/CAD/Product-Bulletins/TN52646-E-0215M-NucleicAcid.pdf#:~:text=A%20low%20A260%2FA230%20ratio%20may,the%20same%20pH%20and%20of. | es_CO |
| dc.relation.references | Nematollahi, A., Decostere, A., Pasmans, F & Haesebrouck, F. (2003). Flavobacterium psychrophilum infections in salmonid fish. Journal of Fish Diseases. Pag 563–574. https://doi.org/10.1046/j.1365-2761.2003.00488.x. | es_CO |
| dc.relation.references | Noga, E. (2010). Fish Disease: Diagnosis and Treatment. Wiley Blackwell. Pag 107-177. https://doi.org/10.1002/9781118786758.ch8. | es_CO |
| dc.relation.references | Ospina, J., Betancur, J., Montoya, A., Correa, L. (2016). PCR Y qPCR PARA DETECCIÓN DE Aeromonas salmonicida Y Flavobacterium psychrophilum EN Oncorhynchus mykis. Revista Investigacion Pecuaria. Recuperado de https://revistas.udenar.edu.co/index.php/revip/article/view/2901/3449. | es_CO |
| dc.relation.references | Park, J., Roh, H., Lee, Y., Lee, J.-Y., Kang, H.-Y., Seong, M. J., Kang, Y., Heo, Y. U., Kim, B. S., Park, C.-I., & Kim, D.-H. (2023). Characterization and Pathogenicity of Flavobacterium psychrophilum Isolated from Rainbow Trout (Oncorhynchus mykiss) in Korea. Microorganisms. https://doi.org/10.3390/microorganisms11102546. | es_CO |
| dc.relation.references | Perera, C., Acevedo, A. (2018). Nuevas tendencias en el diagnóstico de enfermedades virales en los animales. Revista de Salud Animal. Vol. 40, No. 3. Recuperado de https://censa.edicionescervantes.com/index.php/RSA/article/view/990. | es_CO |
| dc.relation.references | Rangdale, R. E., Richards, R. H. & Aldeman, D. J. (1996). Isolation of Cytophaga psychrophila, causal agent of rainbow trout fry syndrome (RTFS) from reproductive fluids and egg surface of rainbow trout (Oncorhynchus mykiss). Bulletin of the European Association of Fish pathologists, 16:63-67. Recuperado de https://www.eafp.org/download/1996-Volume16/Issue%202/1996%20Vol%2016%20No%201_63.pdf. | es_CO |
| dc.relation.references | Starliper, C. (2011). Bacterial coldwater disease of fishes caused by Flavobacterium psychrophilum. Journal of Advanced Research. Volume 2, Issue 2, pag 97-108. Tomado de: https://doi.org/10.1016/j.jare.2010.04.001. | es_CO |
| dc.relation.references | Strepparava, N., Wahli, T., Segner, H., & Petrini, O. (2014). Detection and quantification of Flavobacterium psychrophilum in water and fish tissue samples by quantitative real time PCR. BMC microbiology, 14, 105. https://doi.org/10.1186/1471-2180-14-105. | es_CO |
| dc.relation.references | Tan, S. C., & Yiap, B. C. (2009). DNA, RNA, and protein extraction: the past and the present. Journal of biomedicine & biotechnology, 2009, 574398. https://doi.org/10.1155/2009/574398. | es_CO |
| dc.relation.references | Távara, C., Serrano, E., Tafalla, C., Burga, C. & Llanco, L. (2019). Identificación molecular de Flavobacterium psychrophilum y caracterización de las alteraciones cutáneas en la trucha arcoíris (Oncorhynchus mikyss) procedente de Puno, Perú. Revista de Investigaciones Veterinarias del Perú, 30(4), 1762-1769. https://doi.org/10.15381/rivep.v30i4.17149. | es_CO |
| dc.relation.references | Taylor, P. & Winton, J. (2002). Optimization of Nested Polymerase Chain Reaction Assays for Identification of Aeromonas salmonicida, Yersinia ruckeri, and Flavobacterium psychrophilum, Journal of Aquatic Animal Health, Vol 14, Issue 3. Pag 216–224. https://doi.org/10.1577/1548-8667(2002)014%3C0216:OONPCR%3E2.0.CO;2. | es_CO |
| dc.relation.references | Terova, G., Cattaneo, A. G., Preziosa, E., Bernardini, G., & Saroglia, M. (2011). Impact of acute stress on antimicrobial polypeptides mRNA copy number in several tissues of marine sea bass (Dicentrarchus labrax). BMC immunology, 12, 69. https://doi.org/10.1186/1471-2172-12-69. | es_CO |
| dc.relation.references | Toyama, T., Kita-Tsukamoto, K., & Wakabayashi, H. (1994). Identification of Cytophaga psychrophila by PCR targeted 16S ribosomal RNA. Fish Pathology, 29(4), 271–275. https://doi.org/10.3147/jsfp.29.271. | es_CO |
| dc.relation.references | Urdaci, M. C., Chakroun, C., Faure, D., & Bernardet, J. F. (1998). Development of a polymerase chain reaction assay for identification and detection of the fish pathogen Flavobacterium psychrophilum. Research in microbiology, 149(7), 519–530. https://doi.org/10.1016/s0923-2508(98)80006-5. | es_CO |
| dc.relation.references | Vaibarová, V., & Čížek, A. (2024). Supposed Virulence Factors of Flavobacterium psychrophilum: A Review. Fishes, 9(5), 163. https://doi.org/10.3390/fishes9050163. | es_CO |
| dc.relation.references | Waskiewicz, A & Irzykowska, L. (2014). Flavobacterium spp. – Characteristics, Occurrence, and Toxicity. Encyclopedia of Food Microbiology (2th Edition). Pag 938-942. https://doi.org/10.1016/B978-0-12-384730-0.00126-9. | es_CO |
| dc.relation.references | Wiklund, T., Madsen, L., Bruun, M. S., & Dalsgaard, I. (2000). Detection of Flavobacterium psychrophilum from fish tissue and water samples by PCR amplification. Journal of applied microbiology, 88(2), 299–307. https://doi.org/10.1046/j.1365-2672.2000.00959.x. | es_CO |
| dc.relation.references | Willemsen, D., Cui, R., Reichard, M., Valenzano, D. (2020) Intra-species differences in population size shape life history and genome evolution. eLife 9:e55794. https://doi.org/10.7554/eLife.55794. | es_CO |
| dc.relation.references | Zamora, L., Palacios, A., Palacios, L., Rodriguez, V., Sanchez, L., Dominguez, L., & Fernandez-Garayzabal, J. (2009). Aislamiento y caracterización de flavobacterias en alevines de trucha arcoíris con sintomatología clínica compatible con RTFS. XII Congreso Nacional de Acuicultura. Pag 334-335. Recuperado de https://www.visavet.es/es/aislamiento-y-caracterizacion-de-flavobacterias-en-alevines-de-trucha-arcoiris-con-sintomatologia-clinica-compatible-con-rtfs/34=1023/. | es_CO |
| dc.relation.references | Zamora, V. (2019). Estandarización de las técnicas de Extracción, Cuantificación y Electroforesis de ARN para su incorporación al Manual de Bioquímica Celular y de los Tejidos I. Universidad Nacional Autónoma de Mexico. Recuperado de https://tesiunamdocumentos.dgb.unam.mx/ptd2019/marzo/0787015/0787015.pdf. | es_CO |
| dc.rights.accessrights | http://purl.org/coar/access_right/c_abf2 | es_CO |
| dc.type.coarversion | http://purl.org/coar/resource_type/c_2df8fbb1 | es_CO |
| Aparece en las colecciones: | Medicina Veterinaria | |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| Gutiérrez_2025_TG.pdf | Gutiérrez_2025_TG | 1,26 MB | Adobe PDF | Visualizar/Abrir |
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.