Verificación del método de esterilidad comercial de la Norma Técnica Colombiana 4433 del 2015 aplicando modificación, en matrices UHT, en la pasteurizadora Santo Domingo S.AP.

Abstract

The procedure used to evaluate the commercial sterility of foods in Colombia, in accordance with the "Colombian Technical Standard 4433 of 2015", provides specific guidelines for performing sterility tests on different types of foods. At the Santo Domingo S.A pasteurizer, an adaptation of the method was implemented, going from using tubes (liquid medium) to Petri dishes (solid medium), using UHT matrices that included whole milk (400 ml), chocolate-flavored milk (200 ml ), dairy drink with oats (200 ml) and whole milk cream (200 g). The productivity of the BHI culture medium was carried out in the growth of Geobacillus stearothermophilus ATCC 7953. Subsequently, the standard method of the Colombian Technical Standard 4433 of 2015 and the modified method were applied in order to find out if this modified version meets the same regulatory requirements as the standard methodology of the Colombian Technical Standard 4433 of 2015. Additionally, the repeatability and reproducibility of the methodologies was evaluated. For data analysis, control charts were used, observing that there is no variation in the data between the UHT matrices for each repetition of the techniques and there was no difference between different analysts. The comparison of results for each UHT matrix between the standard method of the commercial sterility standard NTC 4433 of 2015 and its modification met the necessary commercial sterility requirements of the UHT products used. Likewise, the modified technique does not alter the capacity of guarantee the sterility of the products, being effective and a viable alternative to carry out as a commercial sterility method.