Identificación mediante la prueba tamiz de antígeno bufferado para placa (BPA) para diagnóstico presuntivo de Brucella abortus en búfalas del estado de Santa Bárbara de Zulia en la Agropecuaria Bufasur.

Abstract

In this research, the prevalence of brucellosis was evaluated, a disease caused by bacteria of the genus Brucella spp, which affects various animals, including cattle, horses, pigs, goats and sheep. This study specifically focused on the presence of Brucella abortus in domestic water buffaloes (Bubalus bubalis) located in the Bufasur farm. The research adopted a cross-sectional descriptive approach, which included 30 buffaloes who underwent the BPA (Buffered Plaque Antigen) sieve test. During this process, a positive reacting animal was identified. As a confirmatory test, FPA (Polarized Fluorescence) was carried out with the purpose of ruling out the presence of this disease in the recipients who were going to undergo a transfer of highly genetic embryos. Concluding that BPA, although it shows high sensitivity and speed in obtaining results, could generate false positives by not distinguishing between vaccine or infectious antibodies. Therefore, the importance of confirmation using FPA is highlighted to ensure a more accurate interpretation and avoid misunderstandings in the diagnosis.