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dc.contributor.authorForero Hernández, Jesús David.-
dc.date.accessioned2026-10-07T21:52:28Z-
dc.date.available2023-
dc.date.available2026-10-07T21:52:28Z-
dc.date.issued2023-
dc.identifier.citationForero Hernández, J. D. (2023) Determinación de los niveles de malondialdehído en semen fresco bovino mediante el método TBARS inducido y su relación con los parámetros seminales [Trabajo de Grado Pregrado, Universidad de Pamplona]. Repositorio Hulago Universidad de Pamplona. https://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/11556es_CO
dc.identifier.urihttps://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/11556-
dc.descriptionEn el presente estudio se planteó evaluar la concentración de malondialdehído mediante el método de sustancias radioactivas al ácido tiobarbitúrico (TBARS) y cómo se relaciona esta con los parámetros seminales en 14 muestras de semen fresco bovino, el grupo de estudio en su mayoría fue toros de raza Nelore, las muestras se recolectaron por vagina artificial, para su respectivo análisis se realizaron las pruebas de tinción Eosina-Nigrosina, conteo en cámara de Neubauer, análisis espermático asistido por computadora (CASA) y el método TBARS. La peroxidación lipídica espermática se indujo mediante la adición de sulfato de hierro + ácido ascórbico y se determinó su concentración mediante espectrofotómetro (UV-Vis de micro volumen Thermo Scientific™ NanoDrop™ One C) a una longitud de onda de 532 nm. Encontrándose, que los niveles de especies reactivas de oxígeno (ROS) en semen fresco, basados en la medición de TBARS, no están relacionados con la fertilidad de machos reproductores bovinos, dado que no se observaron diferencias estadísticamente significativas (P > 0,05) entre los animales con buena y mala fertilidad. no obstante, se plantea que la medición de la concentración de malondialdehído mediante el método TBARS en corto tiempo no arroja valores de relevancia para su análisis.es_CO
dc.description.abstractIn the present study, it was proposed to evaluate the concentration of malondialdehyde by means of the method of radioactive substances to thiobarbituric acid (TBARS) and how it is related to seminal parameters in 14 samples of fresh bovine semen, the study group was mostly bulls of Nelore breed, the samples were collected by artificial vagina, for their respective analysis the Eosin-Nigrosin staining tests, Neubauer chamber count, computer-assisted sperm analysis (CASA) and the TBARS method were performed. Sperm lipid peroxidation was induced by adding iron sulfate + ascorbic acid and its concentration was determined by spectrophotometer (Thermo Scientific™ NanoDrop™ One C micro-volume UV-Vis) at a wavelength of 532 nm. It was found that the levels of reactive oxygen species (ROS) in fresh semen, based on the TBARS measurement, are not related to the fertility of bovine reproductive males, since no statistically significant differences were observed (P > 0.05). between animals with good and poor fertility. however, it is suggested that the measurement of malondialdehyde concentration using the TBARS method in a short time does not yield relevant values for its analysis.es_CO
dc.format.extent40es_CO
dc.format.mimetypeapplication/pdfes_CO
dc.publisherUniversidad de Pamplona - Facultad de Ciencias Agrarias.es_CO
dc.subjectEstrés oxidativo,es_CO
dc.subjectLipoperoxidación.es_CO
dc.subjectNelore.es_CO
dc.titleDeterminación de los niveles de malondialdehído en semen fresco bovino mediante el método TBARS inducido y su relación con los parámetros seminales.es_CO
dc.typehttp://purl.org/coar/resource_type/c_7a1fes_CO
dc.date.accepted2023-
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dc.rights.accessrightshttp://purl.org/coar/access_right/c_abf2es_CO
dc.type.coarversionhttp://purl.org/coar/resource_type/c_2df8fbb1es_CO
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