Caracterización in silico de sistemas Histidina cinasas de dos componentes en la familia Symbiodiniaceae.

Abstract

The signal transduction pathways are essential for cell function, as well as for the establishment, development, and maintenance of the mutualistic symbiotic relationship Cnidaria-Symbiodiniaceae. Some of the proteins of the two-component Histidine Kinase (HK) family could be part of the signal transduction pathway responsible for the production of glycerol, one of the main photosynthates transferred by Symbiodiniaceae to its cnidarian hosts. These proteins have sensitization domains (DHp and CA) and a response domain (RR) and can act as mechanical receptors, photo- and chemo-receptors in both prokaryotes and eukaryotes (except animals and parasites). This reception characteristic is due to the different evolutionary events that have converged in the formation of HKs proteins since they have gained various protein domains through horizontal transfer, duplication, and gene fusion. Due to the above, histidine kinases, proteins can be found as hybrid proteins (with the addition of a REC receptor domain) or simple proteins made up solely of the sensitization domains. However, the full diversity and structural characteristics of HK proteins in the dinoflagellate microalgae family Symbiodiniaceae are unknown. For this reason, a characterization of simple and hybrid Histidine Kinases proteins was carried out in the Symbiodiniaceae family and the Dinophyceae class with free lifestyles and symbionts. Phylogenies of these proteins were constructed and the phylogenetic relationship between all proteins was analyzed for the presence of conserved domains. Furthermore, using in silico analysis, the subcellular location of these proteins was characterized. In this work, histidine kinase protein sequences obtained from transcriptomes and genomes of dinoflagellate protists of the Symbiodiniaceae family and Dinophyceae class were identified and phylogenetically characterized, finding a prevalence of HKs in microalgae that have a symbiotic relationship with scleractinian cnidarians. Keywords:Signal transduction, histidine kinases, symbiosis, Symbiodiniaceae, in silico.