Producción de xilitol a partir de la fermentación de la xilosa por Saccharomyces Cerevisiae modificada.

Abstract

In the present degree work, the fermentative capacity in the production of xylitol from xylose of the industrial mutant strain Saccharomyces cerevisiae FMYX was evaluated. For this, xylose fermentation tests were carried out, seeking to determine the best conditions for the production of xylitol; the influence of the hydroxymethylfurfural (HMF) and Furfural inhibitors was also evaluated. The results allowed determining that, by allowing initial growth in the presence of oxygen until aerobiosis was reached, at a temperature of 30 ° C, 250 r.p.m and reaching 1 OD of S. cerevisiae culture; they were the best conditions in the production of xylitol from xylose. It was also possible to observe that the presence of inhibitors in the medium affects production. However, the FMYX strain was highly tolerant since it managed to produce xylitol in the presence of different concentrations of the inhibitors. The possible use of the FMYX strain for the production of xylitol, using cane bagasse can be seen as a reality, since, in a fully integrated process, it could represent an advance towards bioproduction, mitigation of chemical production costs and environmental impact.