Diseño e implementación de un sistema automático y de control para el congelamiento de semen Bovino.

Abstract

In the process of freezing of bovine semen (cryopreservation) it is to reduce the temperature of the sample until the point of biological stability. This freezing process is carried out by immersing the semen samples in liquid nitrogen (LN2). When a sample is immersed in liquid nitrogen (LN2) enters a boiling regime due to the large temperature differences between the sample and the liquid nitrogen (LN2) causing evaporation of the liquid nitrogen (LN2) and crystallization Sample abruptly which would affect the entire biological sample. The process of evaporation of liquid nitrogen (LN2) is a phenomenon that occurs because the heat flow from the sample into liquid nitrogen (LN2), in the vicinity of the sample an insulating layer which retards steam energy transfer is generated. This phenomenon is known as effect "Leidenfrost". Cold curves for freezing biological samples of bovine semen are rare in the literature; there are also various rhythms freeze that has been made manually and have taken acceptable results. This study allows us to design and implement an automated system control and freezing of bovine semen that can follow or simulate the curve ideal cold for optimal freezing after stabilization, to achieve this it is necessary to mathematically model the equations describing transfer heat using sampling methods.