Control de calidad de medios de cultivo empleados para determinar la presencia de bacterias patógenas específicas en un laboratorio de microbiología.

Abstract

The quality control of culture media involves a series of tests and evaluations carried out in order to guarantee the efficacy of the culture media used in microbiology laboratories; this includes, among other things, determining its productivity and selectivity, evaluating its physicochemical properties, verifying its sterility. The objective of this study was to evaluate the quality of the culture media Palcam, Hektoen, Burkholderia cepacia agar and Fraser broth for 30 days at the Laser Pharmaceutical S.A.S. Laboratory. Within the results obtained, the productivity of the solid culture media was greater than 90%, their selectivity was verified by not allowing the growth of unwanted strains; for the promotion of Fraser broth, the color change to black was visibly observed when inoculating 100 CFU/mL of the target microorganism and the inhibition of the unwanted strain; all the culture media met the quality standards in the 3 times analyzed (0 days, 15 days and 30 days), therefore, it was established that the validity time of the culture media studied was 30 days when stored in refrigeration. at a temperature of 2°C to 8°C.