Efecto de la translocación de glucosa sobre el metabolismo de EXAIPTASIA DIAPHANA (Phylum: Cnidaria), en estado aposimbiótico y simbiótico.

Abstract

Some corals, animals of the Cnidaria phylum, are characterized by having an association with photosynthetic dinoflagellates of the Symbiodiniaceae family. This symbiotic relationship is the fundamental basis for these animals to be able to live in tropical and subtropical oligotrophic marine waters, and build the colorful and biodiverse coral reef ecosystem. However, its slow growth, its difficulty and high cost of reproduction in captivity, have made it difficult for some experimental studies that address key questions of their symbiotic relationship. For this reason, the Exaiptasia diaphana anemone of the Anthozoa class is one of the best models for the study of symbiosis because it has advantages such as carrying out studies on a genomic scale. In order to better understand their symbiotic interaction, this research relied on Elemental Flow Modes analysis to assess the effects of glucose on the anemone's metabolic network. The aposimbiotic state was taken into account, blocking the entry of glucose and ammonium, and the symbiotic state, with normal flow of these metabolites. The results show that the aposimbiotic condition causes a decrease in the biomass reactions of some non-essential amino acids and carbohydrates, compared to the symbiotic condition. This is due to the fact that the glucose translocated by the symbionts is used by the host to recycle the ammonium and subsequently incorporate it into amino acids. In addition, the deactivation of some reactions involved in the metabolism of Exaiptasia and heterotrophy (obtaining nutrients from external sources) explains the decrease in the metabolic potential of biomass metabolites, essential in processes such as respiration, growth, and reproduction of the host.