Diagnóstico de parámetros técnicos en la empresa Vita IVF LAB para la producción de embriones bovinos in vitro en la ciudad de Bogotá, Colombia.

Abstract

The present internship project focused on evaluating the different processes that the company Vita IVF Lab uses for the production of bovine embryos in vitro, this evaluation was carried out through observation and participation in the different activities carried out by the company.

The embryos are produced from the oocytes obtained by follicular aspiration in live cows on the clients' premises, after the search and classification of the oocytes, these are introduced into cryotubes containing maturing medium so that the oocytes in 24 hours they mature to ovules and after that the ovules are fertilized with bovine semen previously selected by the producer; after 22 hours after fertilizing the eggs, they become known as zygotes and enter the cultivation phase, where the eggs that were correctly fertilized are reviewed and they managed to advance to the zygote stage by passing them to special plates that they contain culture medium that provides the zygote with all the nutrients it needs to continue developing; three days after culture, cleavage is carried out, in this process the main objective is to observe which zygotes uccessfully carried out the divisions in the form of blastomeres and it is called an embryo in its first stages of cell division, these embryos are they separate from those that failed to dividecorrectly and are left in the incubator for three more days until the time of freezing.

At the end of the internship, it was concluded that the processes used by the company make it possible to have a large number of bovine embryos at different stages of development, which are intended for sale fresh or frozen.