Evaluación del efecto de la metformina en la viabilidad espermática de semen ovino refrigerado.

Abstract

Thanks to the growth of sheep production in Colombia, they seek to optimize and improve all the productive and reproductive process of the same, within these processes is the dilution and conservation of sheep semen. In the sheep semen preservation process it is known that there is a loss of up to 50% of the seminal cells, thanks to the fact that commercial diluents do not completely prevent the damage caused by all cryopreservation processes. Bearing in mind that there are studies where metformin has been used to improve semen quality in mice and humans; In this research project 0 mg, 2.5 mg, 5 mg and 10 mg metformin were added and the data were taken at 0, 24, 48 and 72 hours after collection respectively. For the evaluation of motility and progressivity, three fields were analyzed that the software took at random for each time and concentration of the antioxidant, taking an average of 300 sperm, the vitality was determined by the technique of staining with Eosin-Nigrosine per 100 sperm count in a light microscope with 400X magnification, and translucent sperm were classified as alive and partially or totally colored as dead. With this investigation it was possible to determine that motility based on progressivity and speed has favorable results with inclusion levels of 5 mg of metformin until 72 hours, for the variable vitality of sperm cells as time increases the percentages of Survival decreases similarly for each treatment.