Informe final de pasantía profesional en la Universidad de San Paulo.

Abstract

The Bovine embryo in vitro production must be seen as one more alternative to be introduced in the assisted reproduction programs. It has importance to biotechnologies studies and it has been used commercially. The production of embryos in vitro (PIV), has been used commercially for some companies with good results. The primary purpose of PIV commercial seeks to obtain viable embryos of bovine females who are no longer able to produce offspring by conventional techniques, such as donor cows showing infertility caused by treatment with gonadotrophin or by pathological disorders of the reproductive female. Females from six months of age, pregnant women up to the third month, and even post- partum (2 to 3 weeks) can be used as donors for the PIV. Another advantage of follicular aspiration guided by ultrasound in PIV is in the fact that it is not necessary the use of hormones for the recovery of oocytes. Despite these advantages, studies related to the maturation oocitária, oocyte fertilization and embryo culture are still seeking the necessary technical and maximising the production of embryos of good quality. The goal dessse work is to describe the technique and the main steps of the production of embryos in vitro cattle, activities which were applied in the professional practicum carried out at the University of Sao Paulo.