• Repositorio Institucional Universidad de Pamplona
  • Trabajos de pregrado y especialización
  • Facultad de Ciencias Agrarias
  • Medicina Veterinaria
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    dc.contributor.authorMurillo Sanchez, John Camilo.-
    dc.date.accessioned2022-05-09T22:04:23Z-
    dc.date.available2016-11-09-
    dc.date.available2022-05-09T22:04:23Z-
    dc.date.issued2017-
    dc.identifier.citationMurillo Sanchez, J.C.(2016).Informe final de pasantía profesional en la Universidad de San Paulo [Trabajo de Grado Pregrado, Universidad de Pamplona]. Repositorio Hulago Universidad de Pamplona. http://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/621es_CO
    dc.identifier.urihttp://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/621-
    dc.descriptionLa producción in vitro de embriónes (PIVe) bovinos se debe ver como una alternativa para ser introducida en los programas de reproducción, ya que además de su importancia para estudios biotecnológicos posee fundamento comercial. La PIV esta siendo usada comercialmente por algunas empresas, con buenos resultados. El principal objetivo de la PIV comercial se basa en la obtención de embriones viables de hembras que no están aptas para producir descendientes por técnicas convencionales, como en vacas donadoras que presentan infertilidad probada por tratamientos con gonadotropinas o por disfunciones patológicas del aparato reproductor femenino. Hembras a partir de los seis meses de edad, gestantes hasta el tercer mes y vacas pos parto (2 a 3 semanas) pueden ser utilizadas como donadoras para la producción in vitro de embriones. Otra ventaja de las aspiración folicular guiada por ultrasonografía en la PIV es el hecho de que no es necesario el uso de hormonas para la obtención de los ovocitos. A pesar de estas ventajas, estudios relacionados con la maduración ovocitaria, fecundación ovocitaria y cultivo de embriones son todavía necesarios apuntando a la mejora de la técnica junto con la producción de embriones de buena calidad. El objetivo de este trabajo es describir la técnica y las principales etapas de la producción in vitro de embriones, actividades efectuadas en el marco de la pasantía profesional realizada en la Universidad de San Paulo.es_CO
    dc.description.abstractThe Bovine embryo in vitro production must be seen as one more alternative to be introduced in the assisted reproduction programs. It has importance to biotechnologies studies and it has been used commercially. The production of embryos in vitro (PIV), has been used commercially for some companies with good results. The primary purpose of PIV commercial seeks to obtain viable embryos of bovine females who are no longer able to produce offspring by conventional techniques, such as donor cows showing infertility caused by treatment with gonadotrophin or by pathological disorders of the reproductive female. Females from six months of age, pregnant women up to the third month, and even post- partum (2 to 3 weeks) can be used as donors for the PIV. Another advantage of follicular aspiration guided by ultrasound in PIV is in the fact that it is not necessary the use of hormones for the recovery of oocytes. Despite these advantages, studies related to the maturation oocitária, oocyte fertilization and embryo culture are still seeking the necessary technical and maximising the production of embryos of good quality. The goal dessse work is to describe the technique and the main steps of the production of embryos in vitro cattle, activities which were applied in the professional practicum carried out at the University of Sao Paulo.es_CO
    dc.format.extent87es_CO
    dc.format.mimetypeapplication/pdfes_CO
    dc.language.isoeses_CO
    dc.publisherUniversidad de Pamplona – Facultad de Ciencias Agrariases_CO
    dc.subjectEmbriones.es_CO
    dc.subjectMaduración in vitro.es_CO
    dc.subjectFecundación in vitro.es_CO
    dc.subjectUltrasonografía.es_CO
    dc.titleInforme final de pasantía profesional en la Universidad de San Paulo.es_CO
    dc.typehttp://purl.org/coar/resource_type/c_7a1fes_CO
    dc.date.accepted2016-08-09-
    dc.relation.referencesArruda, R. P. Avaliação dos efeitos de diluidores e crioprotetores para o espermatozoide equino pelo uso de microscopia de epiufluorescência, citometria de fluxo, análises computadorizadas da motilidade (CASA) e da morfometria (ASMA). 2000. 121f. Tese (Livre Docência) - Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, 2000.es_CO
    dc.relation.referencesAustin, C. R. (1951). Observations on the penetration of the sperm into the mammalian egg. Australian Journal of Science Research. 4. 581 – 596.es_CO
    dc.relation.referencesBarend, M., Gadella., Luna C. (2014). Cell biology functional dymanics of the mammalian sperm.Theriogenology. 81. 74–84 .es_CO
    dc.relation.referencesBeker-van Woudenberg, A. R., Zeinstra, E. C., Roelen, B. A. J., Colenbrander, B., Bevers, M. M. (2006). Developmental competence of bovine oocytes after specific inhibition of MPF kinase activity: effect of estradiol supplementation and follicle size. Animal Reproduction Science. 92. 231-240.es_CO
    dc.relation.referencesBrogliatti, G. M., Adams, G. P. (1996) Ultrasound-guided transvaginal oocyte collection in prepurbetal calves. Theriogenology. 45. 1163-1176.es_CO
    dc.relation.referencesCaixeta, E. S., Machado, M. F., Ripamonte, P., Price, C., Buratini, J. (2013). Effects of FSH on the expression of receptors for oocyte-secreted factors and members of the EGF-like family during in vitro maturation in cattle. Reproduction Fertility and Development. 25. 890-899.es_CO
    dc.relation.referencesCastro, L. S. (2014). Efeito do estresse oxidativo no espermatozoide e relação com o desenvolvimento embrionário. (Tesis de maestría, Universidade de São Paulo). Recuperado de http://www.teses.usp.br/teses/disponiveis/10/10131/tde 16032015-102623/es.phpes_CO
    dc.relation.referencesCeleghini, E. C. C., Arruda, R. P., Andrade, A. F. C., Nascimento, J., Raphael, C. F. (2007). Practical techniques for bovine sperm simultaneous fluorimetric assessment of plasma, acrosomal and mitochondrial membranes. reprod. domest. anim. 42. 479-488.es_CO
    dc.relation.referencesCesari A, Kaiser GG, Mucci N, et al (2006). Integrated morphophysio- logical assessment of two methods for sperm selection in bovine embryos production in vitro. Theriogenology. 66. 1185–93.es_CO
    dc.relation.referencesChoi, Y. H., Carnevale, E. M., Seidel G. E., Squires, E. L. (2000). Effects of gonadotropins on bovine oocytes matured in TCM-199. Theriogenology. 56. 661-670.es_CO
    dc.relation.referencesColenbrander, B., Brouwers, J. F. H. M., Neild, D. M., Stout, T. A. E., Da Silva, P., Gadella, B. M. (2002). Capacitation dependent lipid rearrangements in the plasma membrane of equine sperm. Theriogenology. 58. 341-345.es_CO
    dc.relation.referencesConnell, M. O., McClure, N., Lewis, S. E. M. (2002). The effects of cryopreservation on sperm morphology, motility and mito- chondrial function. Human Reproduction. 17. 704–709.es_CO
    dc.relation.referencesCross, N. L., Morales, P., Overstreet, J. W., Hanson, F. W.(1986). Two simple methods for detecting acrosome-reacted human sperm. Gamete Research. 15. 213–226.es_CO
    dc.relation.referencesDe Roover, R., Genicot, G., Leonard, S., Bols, P., Dessy, F. (2005). Ovum pick up and in vitro embryo production in cows superstimulated with an individually adapted superstimulation protocol. Animal Reproduction Science. 86. 13-25.es_CO
    dc.relation.referencesFair, T., Hyttel, P., Greve, T. (1995). Bovine oocyte diameter in relation to maturational competence and transcriptional activity. Molecular Reproduction and Development. 42. 437-442.es_CO
    dc.relation.referencesFarrell, P. B., Presicce, G. A., Brockett, C. C., Foote, R. H. (1998). Quantification of bull sperm characteristics measured by computer-assisted sperm analysis (CASA) and the relationship to fertility. Theriogenology. 49. 871-879.es_CO
    dc.relation.referencesGadella, B. M., Luna, C. (2014). Cell biology and functional dynamics of the mammalian sperm surface. Theriogenology. 81. 74-84.es_CO
    dc.relation.referencesGalli, C., Crotti, G., Notari, C., Turini, P., Duchi, R., Lazzari, G. (2000). Embryo production by ovum pick up from live donors. Theriogenology. 55. 1341-1357.es_CO
    dc.relation.referencesGalli, C., Duchi, R., Crotti, G., Turini, P., Ponderato, N., Colleoni, S., …Lazzari, G (2003). Bovine embryo technologies. Theriogenology. 59. 599-616.es_CO
    dc.relation.referencesGarcia, J. M., Avelino, K. B., Vantini, R. (2004). Estado da arte da fertilização in vitro em bovinos. in: simpósio internacional de reprodução animal aplicada, 1, 2004, londrina. São Paulo: biblioteca da Faculdade de medicina Veterinária e Zootecnia da usp, p. 223- 230.es_CO
    dc.relation.referencesGarner, D. I., Thomas, A. C., Joerg, H. W., Dejarnette, J. M., Marshall, C. E. (1997). Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biology of Reproduction. 57.1401-1406.es_CO
    dc.relation.referencesGilchrist, R. B. (2011). Recent insights into oocyte-follicle cell interactions provide opportunities for the development of new approaches to in vitro maturation. Reproduction, Fertility an Development. 23. 23-31.es_CO
    dc.relation.referencesHallap, T., Nagy, S., Jaakma, U., Johannisson, A., Rodriguez- Martinez, H. (2006). Usefulness of a triple fluorochrome combination merocyanine 540/yo-pro 1/hoechst 33342 in assessing membrane stability of viable frozen- thawed spermatozoa from estonian holstein ai bulls. Theriogenology. 65. 1122–1136.es_CO
    dc.relation.referencesHasler, j. f., Henderson, W. B., Hurtgen, P. J., Jin, Z. Q., Mccauley, A. D., Mower, S. A., Neely, B., Shuey, I. S., Stokes, J. E., Trimmer, S. A. (1995). Production, freezing and transfer of bovine ivf embryos and subsequent calving results. Theriogenology. 43. 141 – 152.es_CO
    dc.relation.referencesHazeleger, N.L.; Stubbings, R. B. Developmental potential of selected bovine oocyte cumulus complexes. Theriogenology, v. 37, n. 1, p. 219, 1992.es_CO
    dc.relation.referencesHenkel RR, Schill WB (2003). Sperm preparation for ART. Reprod Biol Endocrinol. 1:108.es_CO
    dc.relation.referencesInternational Embryo Technology Society (IETS, 2014) http://www.iets.org/index.asp?autotry=true&ULnotkn=truees_CO
    dc.relation.referencesInstituto Colombiano Agropecuario (ICA, 2013). http://www.ica.gov.co/Noticias/Pecuaria/2013.aspxes_CO
    dc.relation.referencesKussano, N. R., Lemme, L. O., Guimarães, A. L. S., Franco, M. M., Dode, M. A. N. (2016). Molecular markers for oocyte competence in bovine cumulus cells. Thereogenology 85. 1-10.es_CO
    dc.relation.referencesLee, H. L., Kim, S. H., Ji, D. B., Kim, Y. J. (2013). A comparative study of Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine semen. Jurnal of Veterinary Science. 10. 249-255.es_CO
    dc.relation.referencesLeibfried-Rutledge, M. L., Critserl, E. S., Parrish, J. S., First, N. L. (1989). In vitro maturation and fertilieation of bovine oocytes. Thereogenology. 31. 61-74.es_CO
    dc.relation.referencesLonergan, P., Fair, T. (2016). Maturation of oocytes in vitro. The Annual Review of Animal Biosciences. 4. 10.1-10.12.es_CO
    dc.relation.referencesMachado, G. M., Carvalho, J. O., Filho, E. S., Caixeta, E. S., Franco, M. M., Rumpf, R., Dode, M. A. (2009). Effect of Percoll volume, duration and force of centrifugation, on in vitro production and sex ratio of bovine embryos. Theriogenology. 71. 1289 - 97.es_CO
    dc.relation.referencesMachaty, Z., Peippo, J., Peter, A. (2012). Production and manipulation of bovine embryos: Techniques and terminology. Theriogenology. 78. 937-950.es_CO
    dc.relation.referencesManik, R. S., Singla, S. K., Palta, P. (2003). Collection of oocytes through transvaginal ultrasound-guided aspiration of follicles in an Indian breed of cattle. Animal Reproduction Science. 76. 155–161es_CO
    dc.relation.referencesMerton, J. S., De Roos, A. P. W., Mullaart, E., De Ruigh, L., Kaal, L., Vos, P. L. A. M., Dieleman, S. J. (2003). Factors affecting oocyte quality and quantity in commercial application of embryo technologies in the cattle breeding industry. Theriogenology. 59. 651-674.es_CO
    dc.relation.referencesNedambale, T. L., Dinnyés, A., Groen, W., Dobrinsky, J. R., Tian, X. C., Yang, X. (2004). Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification. Theriogenology. 62. 437-449.es_CO
    dc.relation.referencesNoguchi, M., Yoshioka, K., Hikono, H., Iwagami, G., Suzuki, C., & Kikuchi, K. (2013). Centrifugation on Percoll density gradient enhances motility, membrane integrity and in vitro fertilizing ability of frozen–thawed boar sperm. Zygote. 10. 1 - 8.es_CO
    dc.relation.referencesOliveira, L. Z., Hossepian de Lima, V. F.M., Levenhagen, M. A., Dos Santos, R. M., Assumpção, T.I., Jacomini, J. O., …Beletti, M.E. (2011). Transmission electron microscopy for characterization of acrosomal damage after perrcoll gradient centrifugation of cryopreserved bovine spermatozoa. Journal of Veterinary Science.12. 267-272.es_CO
    dc.relation.referencesOrganización de las Naciones Unidas para la Alimentación y la Agricultura (FAO,2013).http://faostat.fao.org/site/573/DesktopDefault.aspx?PageID=573# ancores_CO
    dc.relation.referencesÕura, C., Toshimori, K. (1990). Ultrastructural studies on the fertilization of mammalian gametes. International Review of Cytology. 122. 105–151. Animal Reproduction Science. 107. 229-236.es_CO
    dc.relation.referencesOshio, S. (1988). Apparent densities of spermatozoa of various mammalian species. Gamete Research. 20. 159-164.es_CO
    dc.relation.referencesPeixer, M. A. S., Rumpf, R., De Bem, A. R., Quiroz, l. M. V. (1997) Produção de embriões e gestações a partir de ovócitos recuperados por ultra-sonografia em fêmeas bovinas superovuladas. in: Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões.es_CO
    dc.relation.referencesPieterse, M. C., Kappen, K. A., Kruip, T. A. M. Taverne, m.a.m. (1987). Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries. Theriogenology. 30. 751 – 762.es_CO
    dc.relation.referencesParrish, J. J. (2013). Bovine in vitro fertilization: In vitro oocyte maturation and sperm capacitation with heparin. Theriogenology. 81. 67-73.es_CO
    dc.relation.referencesParrish, J. J., Susko-Parrish, J. Winer, M. A., First, N. L. (1988). Capacitation of bovine sperma by heparin. 38. 1171 – 1180.es_CO
    dc.relation.referencesPark, J. J., Su, Y. Q., Ariga, M., Law, E., Jin, S. L., Conti, M. (2004). EGF-like growth factors as mediators of LH action in the ovulatory follicle. Journal of Veterinary Science. 303. 682–684es_CO
    dc.relation.referencesPontes, J. H. F., Melo Sterza, F. A., Basso, A. C., Ferreira, C. R., Sanches, B. V., Rubin, K. C. P., Seneda, M. M. (2011). Ovum pick up, in vitro embryo production, and pregnancy rates from a large-scale comercial program using Nelore cattle (Bos indicus) donors. Theriogenology. 75. 1640-1646.es_CO
    dc.relation.referencesQui, M., Yao, Y., Ma, H., Wang, J., Zhao, X., Liu, L., Tang, X., Zgang, L., Sun, F. (2013). Transvaginal Ultrasound-guided Ovum Pick-up (OPU) in Cattle. Biomimetics Biomaterials and Tissue Engineering. 18. 1-3.es_CO
    dc.relation.referencesRenzi, A. (2012). Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos. (Tesis de doctorado). Recuperado de http://www.teses.usp.br/teses/disponiveis/17/17135/tde-19042013- 154338/es.phpes_CO
    dc.relation.referencesRhemrev, J., Jeyendran, R. S., Vermeiden, J. P., Zaneveld, L. J. (1989). Human sperm selection by glass wool filtration and two- layer, discontinuous Percoll gradient centrifugation. Fertil Steril. 51:685–690.es_CO
    dc.relation.referencesRichards, J. S., Russell, D. L., Ochsner, S., Espey, L. L. (2002). Ovulation: new dimensions and new regulators of the inflammatory-like response. Annual Review of Physiology, 64. 69- 92.es_CO
    dc.relation.referencesSutton, M. L., Cetica, P. D., Beconi, M. T., Kind, K. L., Gilchrist, R. B., Thompson, J. G. (2003). Influence of oocyte-secreted factors and culture duration on the metabolic activity of bovine cumulus cell complexes. Reproduction. 126. 27-3es_CO
    dc.relation.referencesSimões, R., Feitosa, W. B., Siqueira, A. F., Nichi, M., Paula-Lopes, F. F., Marques, M. G., …Assumpção, M. E. O. (2013). Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome. Reproduction. 146(5). 433–41.es_CO
    dc.relation.referencesTakagi, M., Kim, I. H., Izadyar, F., Hyttel, P., Bevers, M. M., Dieleman, S. J., Hendriksen, P. J., Vos, P. l. (2001) Impaired final follicular maturation in heifers after superovulation with recombinant human FSH. Reproduction. 121. 941 – 951.es_CO
    dc.relation.referencesVerstegen, J., Iguer-Ouada, M., Oclin, K.(2002). Computer assisted sêmen analyzers in andrology research and veterinary pratice. Theriogenology. 57. 149-179.es_CO
    dc.relation.referencesVaccari, S., Weeks II, J. L., Hsieh, M., Menniti, F. S., Conti, M. (2009). Cyclic GMP signaling is involved in the luteinizing hormone-dependent meiotic maturation of mouse oocytes. Biology of Reproduction. 81. 595-604.es_CO
    dc.relation.referencesWang, Y., Kong, N., Li, N., Hao, X., Wei, K., Xiang, X., …Zhang, M. (2013). Epidermal growth factor receptor signaling-dependent calcium elevation in cumulus cells is required for NPR2 inhibition and meiotic resumption in mouse oocytes. Endocrinology. 10,1210. 2013-1133.es_CO
    dc.relation.referencesYang, X., Jiang, S., Foote, R. H. (1993). Bovine oocyte development following different oocyte maturation and sperm capacitation procedures. Molecular reproduction and development. 34. 94 – 100.es_CO
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