Purificación del PÉPTIDO POLYGLY y evaluación de sus propiedades interfaciales y emulsificantes.

Abstract

The production of biosurfactants represents an alternative in the elaboration of more environmentally friendly products, since they are surfactant molecules obtained on a biological basis. In this study, the experimental emulsifying activity of a peptide designed in a previous study developed at the Universidad de los Andes was evaluated, in which through a rational design based on properties such as polarity, stability, conformations, mass density profiles, Orientations, interaction energy and other properties of amino acids, from molecular dynamics (MD), the PolyGly peptide was selected with one of the sequences with the best ability to stabilize emulsions, to facilitate its subsequent production and purification at the macroscopic level. For this, a recombinant production of the peptide was carried out, it was purified by affinity chromatography and size exclusion, it was lyophilized and its stability and interfacial tension at the water / decane interface were evaluated. Due to the addition of a chain encoding 16 amino acids in the initial plasmid design, the peptide went from weighing 3.72 kDa to 5.37 kDa; This increase generated a change in the properties of the peptide, increasing hydrophobicity, generating emulsifying instability and lack of critical micellar concentration; however, it exhibited a decrease in interfacial tension of 4.64 mN / m for the more concentrated solution (39.65 g / L) by the sloping drop method..