Detección colorimétrica de Plomo y Níquel en aguas utilizando nanopartículas de oro sintetizadas con Quitosán.

Abstract

In the present work a nanobiosensor was developed to be used in the detection of lead and nickel in aqueous solutions of laboratory prepared salts initially the gold nanoparticles were synthesized in aqueous phase using the colloidal method which is part of the bottom-up methods, using tetrachloroauric acid trihydrate as the metallic reagent (HAuCl4 • 3H2O) and chitosan was used as reducing agent, these nanoparticles were functionalized with this same biomolecule. The formation of metallic nanoparticles was confirmed by the Dynamic Light Dispersion technique (DLS) and Scanning Electron Microscopy (SEM) the first one allowed to analyse the size of the synthesized nanoparticles; Simultaneously, the second technique was used to study the other morphological aspects of the itself. The characterization of the nanoparticles was carried out by means of UV-visible absorption and to check the function of the nanosensor against aqueous solutions of lead and nickel salts, colorimetric techniques were used, where the initial color variation of the nanosensor solution indicated the detection of metal by itself, as well as a change generated in the AuNPs spectrum.