Validación de la metodología analítica de valoración microbiológica empleada en el laboratorio AQM S.A.S para cuantificar ESTREPTOMICINA EN materia prima.

Abstract

Antibiotics play a key role in the treatment of bacterial infections. However, it is crucial to use them properly and responsibly to ensure their efficacy and to face the growing problem of bacterial resistance. The main objective of the present work was to validate the analytical methodology for the microbiological quantification of Streptomycin in raw material using the analytical method Agar Diffusion established by the British Pharmacopoeia in its appendix XIV. Streptomycin was selected as the object of analysis and the agar diffusion method was used for its microbiological quantification, being this method established and recognized by the British Pharmacopoeia in its appendix XIV as a reliable and precise technique. During the validation of the analytical methodology, several parameters such as method linearity, system linearity, precision and accuracy, among others, were evaluated. The results obtained are within the range established by the United States Pharmacopoeia. This indicates that the analytical method used is capable of providing accurate and reliable results in the detection of this active principle.