Estandarización de la Técnica de PCR para el diagnóstico de Parvovirosis Canina a partir de muestras de sangre y materia fecal.

Abstract

Canine parvovirus is a disease caused by canine parvovirus (CPV), belonging to the Parvoviridae family and the autonomous parvovirus genus, its origin was due to a mutation in the VP2 protein of the feline panleukopenia virus, developing a variant (CPV2) with the ability to infect canines regardless of their age, puppies being the most prone to contagion, this disease presents with an acute intestinal condition, which has caused a high mortality of canines worldwide. This work was carried out by sampling 27 patients from different veterinary clinics in the city of Pamplona and Cúcuta, with symptoms compatible with canine Parvovirus (vomiting, bloody and non-bloody diarrhea). Of these, 22 presented positive immunochromatography for PCR (rapid test); 4 were negative; and one did not have a snap test. The samples obtained were blood, rectal swab. Initially the DNA extraction methods were standardized using phenol chloroform isoamyl alcohol, boiling snap and a commercial kit. Likewise, through a bibliographical review, previously described primers were selected. The assembly of an end-point PCR and a real-time PCR was carried out, using the different samples and primers.