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    dc.contributor.authorContreras Altahona, Francisco Javier.-
    dc.date.accessioned2024-07-02T21:18:42Z-
    dc.date.available2022-09-01-
    dc.date.available2024-07-02T21:18:42Z-
    dc.date.issued2022-
    dc.identifier.citationContreras Altahona, F. J. (2022). Citotoxicidad de la ocratoxina a sobre la propagación celular de hepg2 [Trabajo de Grado Pregrado, Universidad de Pamplona]. Repositorio Hulago Universidad de Pamplona. http://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/8927es_CO
    dc.identifier.urihttp://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/8927-
    dc.descriptionLa ocratoxina A (OTA) es un metabolito secundario producido por hongos de los géneros Aspergillus y Penicillium, con efecto tóxico para diversos seres vivos, se encuentra en una gran diversidad de alimentos y piensos para animales, por esta razón radica la importancia de estimar los efectos tóxicos de la OTA en la línea celular HepG2 mediante la inhibición de la proliferación celular y determinar los cambios morfológicos en las células y su correlación con la apoptosis. La línea celular HepG2 se expuso durante 48 y 72 horas a concentraciones de 1,0; 2,5; 10 y 15 μM de OTA liofilizada; a continuación, la actividad antiproliferativa de la micotoxina se calculó aplicando el método colorimétrico MTT (bromuro de 3-(4,5-dimetil-2-tiazolil)-2,5-difeniltetrazolio). Posteriormente, en células HepG2 tratadas con 10μM de OTA por 48 horas se analizaron los cambios morfológicos relacionados con la muerte celular apoptótica por microscopia electrónica de transmisión. La propagación de las células HepG2 se afecta desde la concentración de 2,5μM de OTA, en contraste con el control. Después, se realizó la medición de la concentración inhibitoria media (CI50) de OTA sobre las células HepG2, esta fue de 9,19μM DE±0,68 y de 9,98μM DE±0,4, a las 48 y 72 horas, respectivamente. Se evidenciaron alteraciones morfológicas relacionadas con la muerte celular por apoptosis en las células HepG2, como la fragmentación del núcleo (cariorrexis), fragmentación celular y formación de los cuerpos apoptóticos.es_CO
    dc.description.abstractOchratoxin A (OTA) is a secondary metabolite produced by fungi of the Aspergillus and Penicillium genera, with a toxic effect on various living beings, it is found in a wide variety of foods and animal feed, for this reason lies the importance of estimating the toxic effects of OTA on the HepG2 cell line by inhibiting cell proliferation and determining morphological changes in cells and their correlation with apoptosis. The HepG2 cell line was exposed for 48 and 72 hours at concentrations of 1.0; 2.5; 10 and 15 μM lyophilized OTA; next, the antiproliferative activity of the mycotoxin was calculated using the MTT colorimetric method (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide). Subsequently, in HepG2 cells treated with 10μM OTA for 48 hours, the morphological changes related to apoptotic cell death were analyzed by transmission electron microscopy. The propagation of HepG2 cells is affected from the concentration of 2.5 μM of OTA, in contrast to the control. Then, the measurement of the mean inhibitory concentration (IC50) of OTA on HepG2 cells was carried out, this was 9.19μM SD±0.68 and 9.98μM SD±0.4, at 48 and 72 hours, respectively. Morphological alterations related to cell death by apoptosis in HepG2 cells were evidenced, such as fragmentation of the nucleus (karyorrhexis), cell fragmentation and formation of apoptotic bodies.es_CO
    dc.format.extent23es_CO
    dc.format.mimetypeapplication/pdfes_CO
    dc.language.isoeses_CO
    dc.publisherUniversidad de Pamplona - Facultad de Ciencias Básicas.es_CO
    dc.subjectOcratoxina A.es_CO
    dc.subjectProliferación.es_CO
    dc.subjectCitotoxicidad.es_CO
    dc.titleCitotoxicidad de la ocratoxina a sobre la propagación celular de hepg2.es_CO
    dc.typehttp://purl.org/coar/resource_type/c_7a1fes_CO
    dc.date.accepted2022-06-01-
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    dc.rights.accessrightshttp://purl.org/coar/access_right/c_abf2es_CO
    dc.type.coarversionhttp://purl.org/coar/resource_type/c_2df8fbb1es_CO
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