Efecto de la inclusión de concentrado autólogo de plaquetas sobre la fisiología espermática de semen ovino.

Abstract

Plasma rich in platelets (PRP) have been used for healing processes, maxillary oral surgery, arthrology, plastic surgery, although its use in andrology is null; however, there is no information about its effect on sperm motility and morphology. The aim of this study was to compare the effect PRP in inclusion ram semen in the parameters motility and morphometric. Semen samples (n=8) were collected by artificial vagina from two ram located in the North of Santander region, Colombia (L 7°54′N, L 72°30′W). Obtain the blood sample by venous puncture of the jugular the tubes (Citrated and EDTA) centrifuged at 3000 g/3 min, after separation PRP (rich and poor). After the initial evaluation, eppendorf tubes of 2 mL, 500 μL, saline solution, 10 μL of fresh semen and 10 μL PRP (rich or poor) were placed 5 min after preparation. of the samples, (F:fresh, N: saline solution, CPr: citrated platelets rich; CPp: citrated platelets poor; EPr: EDTA platelets rich; EPp EDTA platelets poor). sperm were analyzed per software the Sperm Class Analyser (ISAS, Proiser, Spain) and values in m for length(L), width(W), perimeter(P) and in m2 for area(A), were calculated. Morphometric for Motility (VSL, VCL, VAP. LIN, STR, WOB, ALH, BCF) parameters were compared between treatments (T) by ANOVA. Significant differences (P<0.001) were found between T for morphometric and motility. According to these results, the sperm head dimensions (SHD) of semen samples with inclusion EPr were bigger and the velocity were higher. An increase in SHD has been previously described due to osmolar adjustment procedures, the same happened with the parameter of motility and linearity, improving the characteristic up to 6% with respect to fresh semen. it is observed that the PRP improves the morphometry and the motility of the ram spermatozoa, thanks to the growth factors and the components of its dense granules