Pasantía empresarial en el laboratorio de farmacología vegetal LABFARVE.

Abstract

In this business internship work (April 1 to July 31), LABFARVE laboratory was supported in two very important projects. First, in the quantification of quercetin in the extract of Calendula Officinalis L. (as it is the extract with the highest rotation), by means of the technique of instrumental analysis of high performance liquid chromatography (HPLC), setting the phase mobile (polar), with phosphoric acid 0.08% A, and methanol B, an injection volume of 5 µL, a flow of 1 mL/min, a temperature of 35 °C, and the use of a gradient for optimal elution of the quercetin peak, where a concentration of 5,2180 µg/mL of the marker in the extract was obtained. Second, a preliminary design of a brewer was made, through SolidWorks software, transferring the current process, which is a static process, to a dynamic process, which can optimize the extraction time; finally simulating the operation of the proposed variables for this design. Also, there was a rotation through the different areas that make up the laboratory, developing activities they perform there, and acquiring skills and skills related to the organization and operation of pharmacology laboratories, applying and complementing the knowledge acquired during academic training