Determinación de la presencia de Trypanosoma spp y valoración hematológica en búfalos (Bubalus bubalis) de la Granja Experimental Villa Marina.

Abstract

Buffalo production is primarily developing in tropical and subtropical areas, offering producers an important economic resource due to its increased utilization in these zones. Therefore, in this study, a study was designed to determine the presence of Trypanosoma spp. and perform a hematological assessment in buffaloes (Bubalus bubalis) at the Villa Marina Experimental Farm. This study aims to gather information and monitor hemoparasitic diseases present there. Vital signs such as heart rate, respiratory rate, total blood pressure, temperature, and body condition were recorded. Twenty blood samples were obtained in sterile tubes containing anticoagulant (EDTA) for complete blood counts; for blood smears, samples were taken from the ear in microtubes containing EDTA. Once the results were obtained, conventional PCR was performed on 10 of the animals. Primers H1259 and L1085 were used for 10 samples, and a negative control was used for Trypanosoma spp. DNA identification. Once the results are obtained, the presence of hemoparasites is confirmed through the use of direct and indirect techniques, allowing for the recording and monitoring of these etiological agents that cause disease and poor performance in animals in the short or long term.