Efecto De La Cafeína, Pentoxifilina Y Heparina En La Criopreservación De Semen Equino.

Abstract

Equine semen cryopreservation is a fundamental tool in assisted reproduction programs, allowing stallion fertility to be preserved and artificial inseminations to be performed at optimal times. However, this process can induce structural and functional damage to sperm, affecting their quality. This study evaluated the effect of three molecules—caffeine, heparin, and pentoxifylline—supplemented in the freezing medium on various post-thawing sperm parameters, such as motility, kinetics, and viability. To this end, 10 ejaculates from 5 horses were collected and diluted in Equiplus®, supplemented with caffeine, pentoxifylline, and heparin, and cryopreserved at -196 °C. Once the samples were thawed, sperm motility and kinetics were determined: VE, HOST, and PMM, MT, MP, VSL, VCL, VAP, ALH, BCF, LIN, and STR. Comparisons of means between treatments were performed using the Tukey test. PTX supplementation improved VE. The addition of caffeine reduced MT, MP, and VE, while heparin had no effect. In short, these molecules can improve sperm quality when added at appropriate concentrations.