Producción in vitro de embriones de búfalas en el estado de Santa Bárbara del Zulia – Venezuela.

Abstract

The in vitro embryo production method (PIVE) in buffaloes is a reproductive biotechnology that favors genetic improvement, and in turn contributes to study areas. For the development of this technique, oocytes recovered from ovaries are obtained either by aspiration with guided ultrasonography in live animals or recovered from ovaries of donors from the slaughter plant; these are transported to the laboratory, where the most suitable oocytes are selected, being grade I and grade II, which are subjected to in vitro maturation processes for their subsequent fertilization with spermatozoa that have to go through prior training to later be move to an in vitro culture medium and thus achieve an optimal embryo between days 7 and 8. Through this work, the steps carried out in each stage of the in vitro production of buffalo embryos of the Murrah and Mediterranean breed are described, based on the two methods of follicular aspiration with the adaptation of the media normally used in in vitro production. Of bovine embryos, obtaining as a final result 22.3% of suitable embryos to be transferred from the 286 oocytes initially collected.