• Repositorio Institucional Universidad de Pamplona
  • Trabajos de pregrado y especialización
  • Facultad de Ciencias Agrarias
  • Medicina Veterinaria
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    dc.contributor.authorSalazar Perez, Carlos Andres.-
    dc.contributor.authorBuitrago Lloreda, Oscar Dario.-
    dc.date.accessioned2022-05-16T15:29:06Z-
    dc.date.available2020-09-12-
    dc.date.available2022-05-16T15:29:06Z-
    dc.date.issued2020-
    dc.identifier.citationSalazar Pérez, C. A.; Buitrago Lloreda, O. D. (2020). Efecto de la bursectomía sobre la repuesta inmune (humoral) frente a la enfermedad de Newcastle [Trabajo de Grado Pregrado, Universidad de Pamplona]. Repositorio Hulago Universidad de Pamplona. http://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/782es_CO
    dc.identifier.urihttp://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/782-
    dc.descriptionEn la producción de pollos de engorde, la salud de la parvada tiene un aspecto primordial, en la búsqueda de obtener carne en el menor tiempo posible; la bolsa de Fabricio (BF) es el órgano linfoide encargado de la diferenciación de los linfocitos B, la injuria sobre este órgano representa el desempeño óptimo o no del lote. Con el objeto de evaluar la respuesta inmune humoral frente a la vacuna de Newcastle (ND), se realizó bursectomía total de la bolsa de Fabricio (B) y se compararon los perfiles serológicos utilizando la técnica de inhibición de la hemaglutinación [HI] con pollos no bursectomizados. Así, pollos de la linea ROSS AP fueron distribuidos en 5 tratamientos: T0= pollos sin vacuna y BF; T1= pollos B al día 21 y vacunados el día 22; T2= pollos BF y vacunados el día 22; T3= pollos B al día 28 y vacunados el día 29; T4= pollos BF y vacunados el día 29. Los sueros fueron obtenidos por centrifugación en el laboratorio de Ciencias Biomedicas de la Universidad de Pamplona, y enviadas a un laboratorio comercial (SERVET S.A.S) para la prueba de HI (8 Unidades Hemoaglutinantes [AHU]). Al analizar los resultados obtenidos, los titulos de anticuerpos para la enfermedad de Newcastle, no existieron diferencias significativas entre los diferentes tratamientos, entre las aves bursectomizadas y los controles, tomando en cuenta que las aves Bursectomizadas a los días 21 y 28 (T1 y T3), generaron seroconversión por la prueba de HI en los 35, 42 y 49 días respectivamente. Se puede concluir, que extraer la bolsa de Fabricio a partir del día 21 no compromete la respuesta inmune humoral para la enfermedad de Newcastle. Este hallazgo se explica por la función de la bolsa de Fabricio como órgano de maduración de linfocitos B, los cuales ya maduros empiezan a migrar desde la bolsa de Fabricio a los órganos linfoides secundarios como el bazo y a todos los agregados linfoides distribuidos en el organismo.es_CO
    dc.description.abstractIn the production of broilers, the health of the flock has an essential aspect, in the search to obtain meat in the shortest possible time; bursa of Fabricius (BF) is the lymphoid organ responsible for the differentiation of B lymphocytes, the injury to this organ represents the optimal performance or not of the batch. In order to assess the humoral immune response against the Newcastle vaccine (ND), a total bursectomy of bursa of Fabricius (B) was performed and the serological profiles were compared using the hemagglutination [HI] inhibition technique with chickens. not bursectomized. Thus, chickens from the ROSS AP line were distributed in 5 treatments: T0 = chickens without vaccine and BF; T1 = chickens B on day 21 and vaccinated on day 22; T2 = chickens BF and vaccinated on day 22; T3 = chickens B on day 28 and vaccinated on day 29; T4 = chickens BF and vaccinated on day 29. The sera were obtained by centrifugation in the Biomedical Sciences laboratory of the University of Pamplona, and sent to a commercial laboratory (SERVET S.A.S) for the HI test (8 Hemagglutinating Units [AHU]). When analyzing the results obtained, the antibody titers for Newcastle disease, there were no significant differences between the different treatments, between the bursectomized birds and the controls, taking into account that the birds bursectomized on days 21 and 28 (T1 and T3), generated seroconversion by the HI test at 35, 42 and 49 days, respectively. We can conclude that removing bursa of Fabricius from day 21 does not compromise the humoral immune response for Newcastle disease. This finding is explained by the function of bursa of Fabricius as a maturing organ for B lymphocytes, which when mature begin to migrate from bursa of Fabricius to secondary lymphoid organs such as the spleen and to all lymphoid aggregates distributed in the body.es_CO
    dc.format.extent55es_CO
    dc.format.mimetypeapplication/pdfes_CO
    dc.language.isoeses_CO
    dc.publisherUniversidad de Pamplona – Facultad de Ciencias Agrarias.es_CO
    dc.subjectBursectomía.es_CO
    dc.subjectAnticuerpos.es_CO
    dc.subjectSerología.es_CO
    dc.subjectPollos.es_CO
    dc.subjectVacuna.es_CO
    dc.titleEfecto de la bursectomía sobre la repuesta inmune (humoral) frente a la enfermedad de Newcastle.es_CO
    dc.typehttp://purl.org/coar/resource_type/c_7a1fes_CO
    dc.date.accepted2020-06-12-
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