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  • Facultad de Ciencias Básicas
  • Maestría en Biología Molecular y Biotecnología
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    dc.contributor.authorAldana Bohórquez, Sandra Milena.-
    dc.date.accessioned2022-09-25T02:01:49Z-
    dc.date.available2019-04-24-
    dc.date.available2022-09-25T02:01:49Z-
    dc.date.issued2019-
    dc.identifier.citationAldana Bohórquez, S. M. (2019). Estudio del efecto de la subunidad Gα del subgrupo 1 codificada por aga1 de Acremonium chrysogenum en la respuesta a diferentes tipos de estrés fisiológico [Trabajo de Grado Maestría, Universidad de Pamplona]. Repositorio Hulago Universidad de Pamplona. http://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/2890es_CO
    dc.identifier.urihttp://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/2890-
    dc.descriptionLas proteínas Gα heterotriméricas median la transducción de señales que regulan diversos procesos del desarrollo de los hongos y de la repuesta a estrés ambiental. A. chrysogenum, es el productor de uno de los antibióticos más comercializados en el mundo, la cefalosporina C, y del que poco se conoce sobre sus rutas de señalización, y de su respuesta a condiciones de estrés, por lo que se construyeron alelos que dan una señal constitutivamente activa de la proteína Gα del subgrupo 1, los alelos aga1G42R y aga1Q204L. Para cada alelo se obtuvieron colonias que fueron expuestas a diversos agentes, (KCl, NaCl y Glicerina al 0,5M y 1M; H2O2 50mM y 100mM), se usó la cepa silvestre como control. Los resultados obtenidos se analizaron con pruebas no paramétricas, y sugieren que la proteína Gα heterotrimérica regula negativamente la tolerancia a ambientes hiperosmóticos y oxidativos, haciendo más sensible al hongo a los factores de estrés, lo cual se refleja en la disminución de la tasa de extensión radial. La respuesta a estrés lumínico bajo luz constante, y fotoperiodo 12horas Luz/12 oscuridad, no mostró alteración significativa sobre la tasa de crecimiento e indica una posible ausencia de ritmo circadiano en A chrysogenum.es_CO
    dc.description.abstractLa autora no proporciona la información sobre este ítem.es_CO
    dc.format.extent102es_CO
    dc.format.mimetypeapplication/pdfes_CO
    dc.language.isoeses_CO
    dc.publisherUniversidad de Pamplona – Facultad de Ciencias Basicas.es_CO
    dc.subjectGTPasa heterotrimérica.es_CO
    dc.subjectSubunidad Gα.es_CO
    dc.subjectAcremonium chrysogenum.es_CO
    dc.subjectExtensión hifal.es_CO
    dc.subjectEstrés fisiológico.es_CO
    dc.titleEstudio del efecto de la subunidad Gα del subgrupo 1 codificada por aga1 de Acremonium chrysogenum en la respuesta a diferentes tipos de estrés fisiológico.es_CO
    dc.typehttp://purl.org/coar/resource_type/c_bdcces_CO
    dc.date.accepted2019-01-24-
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